Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: The expression of UHRF2 in human cancers and ICC patients. Notes: ( A ) Microarray data analysis of UHRF2 mRNA in gastric cancer, colon cancer, and hepatocellular carcinoma from the publicly available Oncomine database. ( B ) UHRF2 protein expression in randomly selected ICC tissues and their matched peritumor tissues by Western blot. ( C ) Relative expression of UHRF2 among tumor and peritumor tissues using qRT-PCR. ( D ) Positive UHRF2 was observed primarily in the cytoplasm. Representative UHRF2 and H

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques: Expressing, Microarray, Western Blot, Quantitative RT-PCR, Staining, Real-time Polymerase Chain Reaction, Ubiquitin Proteomics, Immunohistochemistry

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: UHRF2 expression resulted in ICC cell proliferation, invasion, migration, and antiapoptosis. Notes: ( A ) UHRF2 expression was interfered by siRNA and confirmed by Western blot and qRT-PCR. GAPDH was used as internal control. ( B ) Cell counting kit-8 assay was used to assess the ability of cell proliferation. ( C ) Transwell assay was used to show the invasion of ICC cells with different UHFR2 expression in 48 hours. Scale bar =100 μm. ( D ) Wound healing assays showed that inhibition of UHRF2 decreased wound healing compared with control cells. Scale bar =100 μm. ( E ) FCM results indicated that anti-UHRF2 caused acceleration of cell apoptosis. The results are mean ± SD of triplicated independent experiments. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: FCM, flow cytometry; FITC, fluorescein isothiocyanate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICC, intrahepatic cholangiocarcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; UHRF2, ubiquitin-like with PHD and ring finger domains 2.

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques: Expressing, Migration, Western Blot, Quantitative RT-PCR, Control, Cell Counting, Transwell Assay, Inhibition, Flow Cytometry, Real-time Polymerase Chain Reaction, Ubiquitin Proteomics

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: UHRF2 was negatively associated with E-cadherin expression in ICC. Notes: ( A ) High expression of UHRF2 and low expression of E-cadherin, and low expression of UHRF2 and high expression of E-cadherin are shown. Scale bar: 100, 200 μm. ( B ) The relationship between UHRF2 and E-cadherin in ICC patients; the protein expression of UHRF2 was significantly negatively correlated with E-cadherin expression. ( C ) UHRF2 and E-cadherin protein were detected by Western blot analysis. ( D ) Representative immunofluorescent images of UHRF2 and E-cadherin in ICC cell lines are shown. Scale bars =100 μm. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: H

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques: Expressing, Western Blot, Ubiquitin Proteomics

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: Prognostic significance was assessed by log-rank tests and Kaplan–Meier analysis. Notes: ( A – D ) Representative graphs of H

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques: Immunohistochemical staining, Staining, Expressing, Ubiquitin Proteomics

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: Correlations between UHRF2 with clinicopathologic features in 139 ICC patients

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques:

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: Univariate and multivariate analyses of factors associated with recurrence and survival

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques:

Journal: OncoTargets and therapy

Article Title: Overexpression of UHRF2 in intrahepatic cholangiocarcinoma and its clinical significance

doi: 10.2147/OTT.S149361

Figure Lengend Snippet: UHRF2 was negatively associated with E-cadherin expression in ICC. Notes: ( A ) High expression of UHRF2 and low expression of E-cadherin, and low expression of UHRF2 and high expression of E-cadherin are shown. Scale bar: 100, 200 μm. ( B ) The relationship between UHRF2 and E-cadherin in ICC patients; the protein expression of UHRF2 was significantly negatively correlated with E-cadherin expression. ( C ) UHRF2 and E-cadherin protein were detected by Western blot analysis. ( D ) Representative immunofluorescent images of UHRF2 and E-cadherin in ICC cell lines are shown. Scale bars =100 μm. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: H

Article Snippet: Rabbit anti-human UHRF2 monoclonal antibody (1:1,000; Wuhan Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) and rabbit anti-human E-cadherin antibody (1:100; Cell Signaling Technology, Danvers, MA, USA) were utilized to detect the levels of UHRF2 and E-cadherin.

Techniques: Expressing, Western Blot, Ubiquitin Proteomics